Expression and clinicopathologic significance of human achaete-scute homolog 1 in pulmonary neuroendocrine tumors / 中国肺癌杂志

<p><b>BACKGROUND AND OBJECTIVE</b>Human achaete-scute homolog 1 (hASH1) gene plays a critical role in development of the central nervous system, automatic nervous system, adrenal medullary chromaffin cells, thyroid C cells and pulmonary neuroendocrine cells. The aim of this study is to determine hASH1 gene expression in the normal lung tissue and various types of lung tumors, to analyze whether its expression correlated with pulmonary neuroendocrine markers, and to explore the possibility of hASH1 as clinical pathological markers in the neuroendocrine tumors compared with previous neuroendocrine tumor markers.</p><p><b>METHODS</b>hASH1, Chromogranin A, Synaptophysin and CD56 expression were examined in lung tumor specimens (lung inflammatory pseudotumor, squamous cell carcinoma, adenocarcinomas, large cell carcinoma, typical carcinoids, atypical carcinoids, large cell neuroendocrine carcinomas and small cell lung carcinoma and corresponding normal lung specimens) using immunohistochemistry (S-P method). Western blot and reverse transcription polymerase chain reaction (RT-PCR) assay were applied to detect the expressions of hASH1 protein and mRNA in lung cancer tissues.</p><p><b>RESULTS</b>hASH1 expression was positive in 2/16 (12.5%) typical carcinoids, 15/20 (75%) atypical carcinoids, 6/10 (60%) large cell neuroendocrine carcinomas and 31/40 (77.5%) small cell lung carcinoma, respectively, but not in any normal lung tissue (0/10), lung inflammatory pseudotumor (0/49), squamous cell carcinoma (0/30), adenocarcinomas (0/30) or large cell carcinoma (0/20). There was a significant difference in hASH1 expression between typical carcinoids and atypical carcinoids (P < 0.01), but not in large cell neuroendocrine carcinomas and small cell lung carcinoma (P > 0.05). hASH1 expression highly closely correlated with Chromogranin A, Synaptophysin and CD56 expression (P < 0.05).</p><p><b>CONCLUSION</b>hASH1 is a new kind of highly specific markers of pulmonary neuroendocrine tumours, and may be applied to clinical pathology diagnosis of the pulmonary neuroendocrine tumors.</p>

Expression and significance of TSG101, P21 and P300/CBP in squamous cell carcinoma and adenocarcinoma of the lung / 中国肺癌杂志

<p><b>BACKGROUND</b>Tumor susceptibility gene 101 (TSG101) is identified in a random mutagenesis screening for potential tumor suppressors in NIH 3T3 cells. This study is designed to investigate the relationship between sex, age, tumor size, histological type, differentiation, clinical stage and lymphnode metastasis with the expression of TSG101 in human squamous cell carcinoma and adenocarcinoma of the lung, and to analyze whether there is relativity between TSG101, P21 and P300/CBP.</p><p><b>METHODS</b>Immunohistochemical method (SP method) was adopted to detect the expression of TSG101, P21 and P300/CBP proteins in cancer tissues and neighboring noncancerous tissues of 79 cases of human squamous cell carcinoma and adenocarcinoma of the lung. Western blot method was adopted to detect the expression of TSG101 protein in 26 tumor tissues and normal tissues adjacent to cancer.</p><p><b>RESULTS</b>The expression rate of TSG101, P21 and P300/CBP was 59.49% (47/79), 60.76% (48/79) and 56.96% (45/79) respectively. There was no relationship between TSG101 expression and age, sex, tumor size, histological type and P-TNM stage (P > 0.05); otherwise, there was closely relationship between TSG101 expression and cellular differentiation, lymphnode metastasis (P < 0.05). The positive rate of P21 and P300/CBP protein was correlated with the cellular differentiation, lymphnode metastasis and P-TNM stage (P < 0.05). The expression rate of TSG101, P21 and P300/CBP in well and moderately differentiated cells was significantly higher than that in poorly differentiated cells (P < 0.05), and the expression rate of TSG101, P21 and P300/CBP in patients without lymphnode metastasis was significantly higher than that in those with lymph node metastasis (P < 0.01). In the group with lymphnode metastasis and the group without lymphnode metastasis, the positive rate of TSG101 was 48.89% and 73.53% respectively. There were significant differences (P < 0.05) of the expression of TSG101 between the two groups, as well as P21 and P300/CBP. The expression level of TSG101 was positively correlated with P21 (r=0.710) and P300/CBP (r=0.689). The expression of TSG101 protein in tumor tissues was significantly lower than that in the neighboring noncancerous tissue.</p><p><b>CONCLUSIONS</b>The expression of TSG101, P21 and P300/CBP protein might be associated with lung cancer differentiation and metastasis, the expression of them is positively correlated with each other, hence they can be used as valuable biomarkers to evaluate lung cancer metastasis.</p>

Expression and its significance of Pin1 and β-catenin in squamous cell carcinoma and adenocarcinoma of the lung / 中国肺癌杂志

<p><b>BACKGROUND</b>The conformation of a subset of phosphorylated serines or threonines preceding proline motifs is regulated by the prolyl isomerase Pin1. Pin1 plays a critical role in oncogenesis. The aim of this study is to explore the relationship between the expression of Pin1 and clinicopathological factors in squamous cell carcinoma and adenocarcinoma of the lung, and to analyze the correlation between Pin1 and β-catenin.</p><p><b>METHODS</b>The expression of Pin1 and β-catenin proteins was detected in 69 lung cancer cases by immunohistochemical SP method, and in 30 fresh lung samples by Western blot.</p><p><b>RESULTS</b>Immunohistochemically, the overexpression of Pin1 and β-catenin in lung cancer was 78.3% (54/69) and 63.8% (44/69), respectively. The expression of Pin1 and β-catenin was not related to age, sex, histological classification, differentiation, lymph node metastasis and pTNM stages. There was a positive correlation between overexpression of Pin1 and aberrant β-catenin expression (P < 0.05). Western blot results showed that the expression of Pin1 and β-catenin in lung cancer tissues was significantly higher than that of paracancerous lung tissues (P < 0.05).</p><p><b>CONCLUSIONS</b>Pin1 is overexpressed in squamous cell carcinoma and adenocarcinoma of the lung and may play a critical role in oncogenesis of lung cancer. Overexpression of Pin1 might contribute to the upregulation of β-catenin and it may be one of the pathways for Pin1 to work.</p>

Expression of integrin-linked kinase and E-cadherin in non-small cell lung cancer / 中国肺癌杂志

<p><b>BACKGROUND</b>Integrin-linked kinase (ILK) is a Ser/Thr protein kinase. Many studies have showed that ILK was closely related to occurrence, proliferation, invasion and metastasis in many malignant tumors, and it appeared to be an upstream cross point of tumor-associated factors. The aim of this study is to explore the relationship between the expression of ILK and some clinical pathological factors in human non-small cell lung cancer (NSCLC), and analyze whether there is relativity between ILK and E-cadherin.</p><p><b>METHODS</b>Immunohistochemical S-P method was adopted to detect the expression of ILK and E-cadherin proteins in 76 NSCLC cases with the neighboring noncancerous tissue, and the expressions of them in 30 fresh NSCLC samples were determined with Western Blot assay.</p><p><b>RESULTS</b>Immunohistochemically, the overexpression of ILK protein in NSCLC was 53/76 (69.7%), including 33/44 (75.0%) squamous cell carcinoma and 20/32 (62.5%) adenocarcinoma, but its expression was not related to the histological type (P= 0.247 ). Expression of ILK was related to differentiation (rs=-0.296, P=0.009), lymph node metastasis (rs=0.311, P=0.006) and clinical stage (rs=0.350, P=0.002). Moreover, Kaplan-Meier survival estimates showed a significant correlation between ILK expression and patient survival in Log-rank test (P=0.006). Overexpression of ILK in NSCLC was associated with unfavorable prognosis. An inverse correlation between the levels of ILK and E-cadherin was found (rs=-0.514, P < 0.001). Western Blot result showed that the level of ILK in the tumor tissues was noticeably higher than that in the normal tissues (t=-6.811, P=0.0002), and an inverse correlation between the levels of ILK and E-cadherin was proved (P=0.001).</p><p><b>CONCLUSIONS</b>In NSCLC, ILK can interact with some tumor-associated factors, through which it appears to be involved in several oncogenesis-related events ,including promotion of cell survival ,as well as cell migration and invasion.ILK keeps significant inverse correlation to E-cadherin,andit would be one of the pathways for ILK to affect differentia-tion,clinical stage ,lymph node metastasis and prognosis of patients .ILK expression can be a useful predictorof poor prognosis in NSCLC,and the detections of ILK and E-cadherin will help us better to predict prognosisof patients .</p>

Value of intraoperative cholangiopancreatography for radical resection of congenital choledochal cyst / 中国普通外科杂志

Objective To evaluate intraoperative cholangiopancreatography in understanding pathologic changes of congenital choledochal cyst(CCC) with anomalous junction of pancreaticobiliary duct(APBD) and for rational operative procedure. Methods Eighty-two CCC cases were examined by intraoperative cholangiopancreatography(ICP). The morphologies of the biliary tract and types of APBD were recorded. Results Of the 82 CCC cases there were 35 cases of Todani Ia,9 cases of Ib,28 cases of Ic and 10 cases of Ⅳ.APBD was diagnosed in 73 cases and classified according to Komi classification. Of them,37 cases were classified as type I of APBD (bile duct drains into pancreatic duct),30 cases as type II (pancreatic duct drains into bile duct) and 6 cases as type III (complicated APBD with patent accessory pancreatic duct). Of the 44 cases with choledochal cystic dilatations,33 cases were of type I. Of the 29 biliary fusiform dilatations,21 were of type II. Of 21 patients with pancreatitis,17 were of type II (? 2=33.14,P